An exsanguinous metabolic support system for maintaining an organ or tissue at a near normal metabolic rate is disclosed. The system employs an organ chamber comprising an oxygenator in the perfusion fluid paths for controlling respiratory gases. The oxygenator may contain three pouches for gaseous exchange of the perfusion solution. A controlled gassing subsystem for regulating respiratory gases and maintaining the pH of the perfusion solution is also employed by the system and delivers oxygen and carbon dioxide to the pouches of the oxygenator. The organ chamber additionally includes a perfusion subsystem including the perfusion fluid paths, a container for holding the organ in the perfusion fluid paths with one or more perfusion solution inlets. The organ chamber may additionally include a conduit for receiving venous outflow of perfusion solution and preventing its contact with the outer surfaces of the organ.
