The invention provides compositions containing hemoglobin, particularly PEGylated hemoglobin. The PEGylated hemoglobin molecule is capable of transferring oxygen or carbon monoxide bound thereto to a tissue with which it is in contact. Exemplary PEGylated hemoglobin formulations of the invention are virally inactivated. Various compositions of the invention include deoxygenated hemoglobin, which may be conjugated with one or more water-soluble polymer. Deoxygenated PEGylated hemoglobin includes those species in which the iron atom of the hemoglobin molecule does not bind oxygen or any other species, and hemoglobin molecules in which a species other than oxygen, e.g., carbon monoxide, is bound to the iron atom. The compositions of the invention are formulated as hypo-, iso- or hypertonic solutions of the PEGylated hemoglobin. The compositions are of use to treat and/or ameliorate disease, injury and insult by providing for the oxygenation of tissues and/organs.本發明提供含血紅素之組成物，尤其是聚乙二醇化血紅素。聚乙二醇化血紅素分子可將與其結合之氧或一氧化碳輸送至其所接觸之組織。例示之本發明聚乙二醇化血紅素調配物係經病毒失活。本發明之各種組成物係包括脫氧血紅素，其可與一或多種水溶性聚合物軛合。脫氧聚乙二醇化血紅素包括其中血紅素分子之鐵原子不結合氧或任何其他類型物質的類型物質，及其中除氧之外的類型物質(例如一氧化碳)結合於鐵原子的血紅素分子。本發明組成物係調配成聚乙二醇化血紅素之低張、等張或過張溶液。該等組成物係藉由提供組織及/或器官之氧合而用以治療及/或改善疾病、損傷及傷害。
