Freddie Van De Velde;Aart Cornelis Alting;Theodorus Arnoldus Gerardus Floris;Igor Bodnár;Fanny Chantal Jacqueline Weinbreck;Jeroen Grandia
发明人:
Aart Cornelis Alting,Theodorus Arnoldus Gerardus Floris,Fanny Chantal Jacqueline Weinbreck,Jeroen Grandia,Freddie Van De Velde,Igor Bodnár
申请号：
US12601114
公开号：
US20130183357A1
申请日:
2008.05.21
申请国别(地区):
US
年份:
2013
代理人:
摘要：
The present invention provides a method of producing a protein-based encapsulate, said method comprising: providing an aqueous solution of a protein that is capable of forming disulfide cross-links； submitting said aqueous solution to a protein activation treatment to produce an aqueous suspension of activated protein aggregates, said suspension having a reactivity of at least 5.0 μmol thiol groups per gram of activated protein aggregates as determined in the Ellman's assay； dispensing said aqueous suspension in a gas or a water-immiscible liquid to produce suspension droplets having a diameter of 0.1-500 μm； and forming disulfide cross-links between the activated protein aggregates by contacting the activated protein aggregates with an oxidizing agent, optionally after said activated protein aggregates have been partially cross-linked by forming disulfide cross-links by means of heat treatment or by pressurization to a pressure in excess of 50 MPa. The aforementioned method offers the advantage that the characteristics of the protein-based encapsulation matrix can be controlled effectively. Furthermore, said method enables the preparation of protein-based encapsulates that very effectively protect the encapsulated components, e.g. against oxidation or moisture.
