Priya KARMALI,Padmanabh Chivukula,Joseph E. Payne,Yanjie Bao
申请号：
US16823212
公开号：
US20200297634A1
申请日:
2020.03.18
申请国别(地区):
US
年份:
2020
代理人:
摘要：
A method of producing a lipid-encapsulated RNA nanoparticle, comprising the steps a) flowing an aqueous solution comprising an RNA through a 1st tube having an inner diameter (ID) of between about 0.1″； and 0.132″；； b) flowing an ethanol solution comprising lipids through a 2nd tube having an ID of between about 0.005″； and 0.02″； at one third the flow rate of the aqueous solution through the 1st tube, wherein the lipids comprise a cationic lipid； and c) mixing the ethanol solution with the aqueous solution by flowing the ethanol solution and the aqueous solution into a mixing module consisting of the 2nd tube perpendicularly joined to the 1st tube； wherein the mixing produces an output solution flowing in the 1st tube comprising a turbulent flow of the RNA and the lipids in between about 10% to 75% ethanol v/v, and wherein the lipid-encapsulated RNA nanoparticles have a bilayer structure.
