Hydroxy phenyl pyruvate dioxygenases (HPPD) derived from plants and resistant to tricetonic herbicides, and transgenic plants containing these dioxygenases
HAWKES, TIMOTHY ROBERT,WARNER, SIMON,ANDREWS, CHRISTOPHER JOHN
申请号：
ES10178572
公开号：
ES2538471T3
申请日:
2001.11.14
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
A method of selecting a polynucleotide encoding a tricetonic herbicide resistant HPPD enzyme comprising screening a population of sequences encoding an HPPD enzyme and selecting as sequences encoding a tricetonase resistant HPPD enzyme those sequences encoding an enzyme that, in comparison with a control HPPD enzyme it has at least an increased resistance 2.5 or, preferably, four times to tricetonic herbicides selected from herbicides having one of the following formulas ** Formula ** and its agrochemically acceptable salts, where Ar A, B, D and E groups are independently selected from optionally substituted phenyl or optionally substituted heteroaryl, where optional substituents for groups A, B, D and E include C1-C4 alkyl, C3-C6 cycloalkyl, halogen, OH, SH, CN, -NH2, -NHCOR, -CONHR, - COR, -SR, SOR, -SO2R, NHR-SO2R, -CO2R, -NO2, CF3, -SF5, OR and CO2H, where R>； = C1-C6 alkyl or aryl optionally substituted with one or more substituents selected from the group consisting of halo or C1-C4 alkoxy, where R1 or R2, or both, are H and both R3 and R4 are H and R5 or R6, or both are H, where both R8 and R9 are H and R10 or R11, or both, are H, where R12 or R13, or both, are H and R14 or R15, or both, are H and where R1-R17 is selected each individually from the group consisting of H, C1-C4 alkyl, C3-C6 cycloalkyl, halogen, OH, SH, CN, -NH2, -NHCOR, -CONHR, -COR, -SR, SOR, -SO2R, NHR- SO2R, -CO2R, -NO2, CF3, -SF5, OR and CO2H in which R>； = C1-C6 alkyl or aryl optionally substituted with one or more substituents selected from the group consisting of halo or C1-C4 alkoxy； in comparison with herbicides selected from the group of herbicides having the formula ** Formula ** and their agronomically acceptable salts, where R1-R6 are each individually selected from the group consisting of H, C1-C4 alkyl, C3-C6 cycloalkyl, halogen, OH, SH, CN, -NH2, -NHCOR, -CONHR, -COR, -SR, SOR, -SO2R, NHR-SO2R, -CO2R, -NO2, CF3, -SF5, OR and CO2H, in which R>； = C1-C6 alkyl or aryl option
