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RNA INTERFERENCE MEDIATING SMALL RNA MOLECULES
专利权人:
MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.
发明人:
WINFRIED LENDECKEL,THOMAS TUSCHL,MATTHIAS WILM,REINHARD LÜHRMANN,SAYDA ELBASHIR
申请号:
PT10180025
公开号:
PT2348134E
申请日:
2001.11.29
申请国别(地区):
PT
年份:
2014
代理人:
摘要:
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3 ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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中国工程科技知识中心
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