Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.;Europäisches Laboratorium für Molekularbiologie
发明人:
ELBASHIR, Sayda,LENDECKEL, Winfried,WILM, Matthias,LÜHRMANN, Reinhard,TUSCHL, Thomas
申请号：
DK01985833
公开号：
DK1407044T4
申请日:
2001.11.29
申请国别(地区):
DK
年份:
2017
代理人:
摘要：
Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
