A dry animal-derived collagen fiber tissue material and preparation method and bioprosthesis thereof are disclosed. The preparation method includes: 1) rinsing of an animal-derived collagen fiber tissue material that has been treated with a crosslinking agent； 2) immersion of the rinsed tissue material in a non-aqueous alcoholic solution for dehydration； 3) successive immersion of the tissue material that has been dehydrated with the non-aqueous alcoholic solution in saccharide solutions of different gradients of concentrations for gradient dehydration； 4) taking out and drying of the gradient dehydrated tissue material； and 5) hermetic packaging of the dried tissue material and sterilization. The preparation method is simple and allows the use of easily-available low-cost materials, resulting in lower costs. In addition, it can reduce the toxicity caused by a residue of the aldehyde crosslinking agent. The prepared biological tissue material is pliable and tough, less prone to bending or warping, has a water content that can be well controlled and better biocompatibility due to absence of polyhydric alcohol residues.
