Stephen Thomas Hadfield;Paul Anthony Thompson;Yan Zhang;Timothy Robert Hawkes;Russell Viner;Glynn Mitchell
发明人:
Timothy Robert Hawkes,Glynn Mitchell,Stephen Thomas Hadfield,Paul Anthony Thompson,Russell Viner,Yan Zhang
申请号:
US12111364
公开号:
US08642836B2
申请日:
2008.04.29
申请国别(地区):
US
年份:
2014
代理人:
摘要:
A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterized in that (i) the non-phytotoxic substance is a D-alpha amino acid, and (ii) the enzyme is a D-amino acid oxidase.