HAWKES, TIMOTHY ROBERT,MITCHELL, GLYNN,HADFIELD, STEPHEN THOMAS,THOMPSON, PAUL ANTHONY,VINER, RUSSELL,ZHANG, YAN
申请号：
CA2475485
公开号：
CA2475485C
申请日:
2003.02.14
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
A method of producing male or female sterile plants comprising thesteps of transforming plant material with a polynucleotide which encodes atleastone enzyme which reacts with a non-phytotoxic substance to produce aphytotoxicone, and regenerating the thus transformed material into a plant, wherein thesaidnon-phytotoxic substance is applied to the plant up to the time of male orfemalegamete formation and/or maturation, so that the non-phytotoxic substanceprovides for the production of a phytotoxic one which selectively prevents theformation of or otherwise renders the said gametes non-functional, wherein theenzyme is expressed preferentially in either male or female reproductivestructures, characterised in that (i) the non-phytotoxic substance is selectedfromthe group consisting of D-alpha amino acids and peptide derivatives of non-proteinD-alpha acids, and (ii) the enzyme is a D-amino acid oxidase.
