Method for producing a three-dimensional cultured skin model including a dermal layer and an epidermal layer and a three-dimensional cultured skin model produced thereby
The present invention relates to a method for preparing a three-dimensionally cultured skin model comprising dermis and epidermis, which comprises: a step of preparing the dermis using a composition comprising murine fibroblasts； and native collagen or a combination of native collagen and atelocollagen； and a step of forming the epidermis using keratinocytes. Also, the present invention relates to a three-dimensionally cultured skin model which comprises: a dermis prepared by a composition comprising murine fibroblasts, native collagen, or a combination of native collagen and atelocollagen； and epidermis formed from keratinocytes. The three-dimensionally cultured skin model of the present invention may be used widely in toxicity and efficacy experiments of medicines or cosmetics, and in the field of alternative experiments for animal experiments since the three-dimensionally cultured skin model is excellent in formation and differentiation of dermis and epidermis using murine 3T3 cells for preparing the skin model and a mixture of atelocollagen and native collagen, and has a structure similar to the human skin layer by inhibiting dermal contraction and collagen degradation in the dermis.
