Bukhtiyarov Igor Valentinovich (RU),Бухтияров Игорь Валентинович (RU),Kuzmina Lyudmila Pavlovna (RU),Кузьмина Людмила Павловна (RU),Khotuleva Anastasiya Grigorevna (RU),Хотулева Анастасия Григорьевна
申请号：
RU2019123116
公开号：
RU0002722264C1
申请日:
2019.07.22
申请国别(地区):
RU
年份:
2020
代理人:
摘要：
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely to toxicology, and can be used for prediction of risk of intoxication with lead and its compounds. Venous blood is sampled, genetic material is recovered, polymerase chain reaction with specific primers is performed, and MspI ALAD polymorphism is detected in dehydrase gene of δ-aminolevulinic acid. Additionally, the amplification products are analyzed by real-time fluorescence detection method, and based on this, detection of CPOX A/C polymorphism in the gene of the coproporphyrinogen oxidase CPOX. If observing the unfavorable allele MspI ALAD*2 dehydratase gene δ-aminolevulinic acid and the unfavorable allele C of the coproporphyrinogen oxidase gene, a high risk of lead intoxication and its compounds in the individuals exposed to lead and its compounds is predicted.EFFECT: method provides more reliable prediction of the risk of developing intoxication lead and its compounds in individuals exposed to lead and its compounds during preliminary and periodic medical examinations of a large number of workers for short (compressed) time, by detecting individuals with a polymorphous version of the dehydratase gene δ-aminolevulinic acid and the possibility of timely detection of individuals with due allowance for their genetic predisposition to disruption of the synthesis of the enzyme coproporphyrinogen oxidase.1 cl, 2 exИзобретение относится к медицине, а именно к токсикологии, и может быть использовано для прогнозирования риска развития интоксикации свинцом и его соединениями. Осуществляют забор венозной крови, выделение генетического материала, проведение полимеразной цепной реакции со специфическими праймерами и выявление полиморфизма MspI ALAD в гене дегидратазы δ-аминолевулиновой кислоты. Дополнительно проводят анализ продуктов амплификации методом флуоресцентной детекции в режиме реального времени и на основании этого выявление полиморфизма СРОХ А/С в гене копропорфириноген оксидазы СРОХ. При одновремен
