Methanoarchaeal lysine 2, 3-aminomutase genes from marineMethanosarcina mazeiN2M9705, halotolerantMethanocalculus chunghsingensisK1F9705b and halophilicMethanohalophilus portucalensisFDF1 are used in the synthesis of pharmacological active compounds. Due to the high salt, organic solvent and drought resistant nature for halotolerant and halophilic organisms, the lysine 2, 3-aminomutase from halophilic methanogens are more stable than that of normal microorganism for use in β-lysine production. Furthermore, methods for inducing β-lysine production in methanoarchaea in a condition of an elevated salt concentration are provided. Therefore, cost and time of production of β-lysine for synthesis of β-lysine associated drugs is reduced.本發明係關於源自可生長於嚴苛環境下之甲烷古菌的賴胺酸2,3-氨基轉位酶酵素及其基因於藥物合成上之應用。由於耐鹽與嗜鹽的微生物蛋白具有耐鹽、耐溶劑與耐低水活性之特質,源自於耐鹽與嗜鹽甲烷古菌之賴胺酸2,3-氨基轉位酶應較一般微生物酵素更穩定。本發明可降低β-賴胺酸(β-lysine)相關之合成藥物的製造成本,且由於可不需進一步純化所得之β-賴胺酸產物因而可縮短製程。
