Single injection systems and methods to obtain parallel tissue conductances within luminal organs. In at least one embodiment of a single solution injection method to obtain a parallel tissue conductance within a luminal organ of the present disclosure, the method comprises the steps of introducing at least part of a detection device into a luminal organ at a first location, the detection device having a detector, applying current to the detection device using a stimulator, introducing a first signal having a first frequency and a second signal having a second frequency through the detection device, and injecting a solution having a known conductivity into the luminal organ at or near the detector of the detection device. Such a method may further comprise the steps of measuring an output conductance of the first signal and the second signal at the first location using the detector, and calculating a parallel tissue conductance at the first location based in part upon the output conductance and the conductivity of the injected solution.
