VAN OPDORP NATHAN,CHANNABASAVARADHYA CHANDRA-SHEKARA,KUMPATLA SIVA P.,WEI CHEN
申请号：
NZ60241811
公开号：
NZ602418A
申请日:
2011.05.11
申请国别(地区):
NZ
年份:
2015
代理人:
摘要：
Disclosed is a method for determining the zygosity and/or presence/absence of an allele using com plant tissue, the method comprising: obtaining a sample of isolated genomic DNA from the corn plant tissue； contacting the isolated genomic DNA with at least one nucleic acid molecule comprising a nucleotide sequence capable of hybridizing to SEQ ID NO: 10 under high stringency conditions and at least one nucleic acid molecule capable of hybridizing to SEQ ID NO: 11 under high stringency conditions； using amplification methods and the detection of first and second reporters, and determining zygosity of a bm3 mutation in the isolated genomic DNA.
