ЧЭНЬ Вэй (US),ВАНОПДОРП Натан (US),ПЛЕН Стив Дж. (US),ФРИДЕМАНН Питер (умер),ЧАИДИР Надия (US),КУМПАТЛА Сива Прасад (US)
申请号：
RU2014152692
公开号：
RU2014152692A
申请日:
2013.05.29
申请国别(地区):
RU
年份:
2016
代理人:
摘要：
1. A method for determining the zygosity and / or presence / absence of an allele with the plant tissue, the method comprising: obtaining a sample of genomic DNA extracted from corn plant tissue actuation isolated genomic DNA into contact with at least one nucleic acid molecule comprising a nucleotide a sequence capable of hybridizing to SEQ ID NO: 9 under highly stringent conditions, and at least one nucleic acid molecule capable of hybridizing to SEQ ID NO: 10 under highly stringent conditions and determining zygosity fl2 mutations in isolated genomic DNK.2. The method of claim 1, further comprising: a. Adduction isolated genomic DNA is contacted with two nucleic acid molecules, each of which comprises a nucleotide sequence capable of hybridizing to SEQ ID NO: 9 under highly stringent conditions, and two nucleic acid molecules capable of hybridization with SEQ ID NO: 10 under highly stringent conditions, amplification of a nucleotide sequence isolated genomic DNA located between the nucleotide sequence of the isolated genomic DNA that hybridize with two molecules nucleic acids, each of which comprises a nucleotide sequence capable of hybridizing to SEQ ID NO: 9 under highly stringent conditions, amplification of a nucleotide sequence isolated genomic DNA located between the nucleotide sequences of isolated genomic DNA that hybridize with two nucleic acid molecules, each of which comprises a nucleotide sequence capable of hybridizing to SEQ ID NO: 10 under highly stringent conditions, included in the amplification reaction of at least one mol1. Способ для определения зиготности и/или наличия/отсутствия аллеля с применением растительной ткани, при этом способ содержит:получение образца выделенной геномной ДНК из растительной ткани кукурузыприведение выделенной геномной ДНК в контакт по меньшей мере с одной молекулой нуклеиновой кислоты, содержащей нуклеотидную последовательность, способную к гибридизации с SEQ ID NO: 9 в очень жестких условиях, и по меньшей мере одн
