TEST-SYSTEM FOR CHEMICAL COMPOUNDS SCREENING FOR INHIBITING ACTIVITY AGAINST PARP-1

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专利权人:

Federalnoe gosudarstvennoe byudzhetnoe obrazovatelnoe uchrezhdenie vysshego obrazovaniya "Moskovskij gosudarstvennyj universitet imeni M.V. Lomonosova" (MGU)

发明人:

Studitskij Vasilij Mikhajlovich,Feofanov Aleksej Valerevich,Sultanov Daniel Chingizovich,Kudryashova Kseniya Sergeevna,Gerasimova Nadezhda Sergeevna,Malyuchenko Nataliya Valerievna

申请号：

RU20160148005

公开号：

RU2649767(C1)

申请日:

2016.12.07

申请国别(地区):

俄罗斯

年份:

2018

代理人:

摘要：

FIELD: medicine.SUBSTANCE: group of inventions concerns a test system for chemical compounds screening for inhibitory activity against PARP-1 based on FRET microscopy of single particles (spFRET), which is a mixture comprising a mononucleosome complex on a nucleosome-positioning DNA template labeled with a pair of fluorophores, forming donor-acceptor pairs to implement the FRET effect; PARP-1 protein and its substrate - NAD+. The group of inventions also relates to a method for chemical compounds screening for inhibitory activity against PARP-1 based on FRET microscopy of single particles.EFFECT: group of inventions provides detection of compounds directed to PARP-1 and capable of inhibiting its activity.12 cl, 1 ex, 8 dwg

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