PROBLEM TO BE SOLVED: To provide a method for promptly and simply measuring the microorganism in a sample including protein as live bacteria in measuring the microorganism count of a sample including protein, though especially in the food sample basically having emulsion structure such as milk and soybean milk, when the emulsion is solubilized and measured by the surfactant or the like, the microorganism can be measured only as the killed bacteria.SOLUTION: After the sample including protein is subjected to deproteinization by isoelectric precipitation, filtration purification liquid including the microorganism is recovered, and live bacteria dyeing is performed using fluorescent reagent. Further, if necessary, the sample is cultured and grown in advance, and after the microorganism in the cultured sample is grown to be detectable bacterial count, live bacteria dyeing is performed using the above fluorescent reagent. Thus, the microorganism in the sample including protein can be measured as live bacteria more than simply as compared with the conventional method.COPYRIGHT: (C)2013,JPO&INPIT【課題】蛋白質を含む試料の微生物数の測定において、特に牛乳や豆乳などのエマルジョン構造を基本とする食品試料では界面活性剤等によりエマルジョンを可溶化して測定すると死菌としてのみしか測定できなかったが、蛋白質を含む試料の微生物を生菌として、迅速に、また簡易に測定する方法を提供する。【解決手段】蛋白質を含む試料を等電点沈殿による除蛋白を行った上で微生物を含んだろ過精製液を回収し、蛍光試薬による生菌染色を行う。また必要により、事前に試料を培養増殖し、該培養後試料中の微生物を検知可能な菌数にまで増殖させた後、前記蛍光試薬による生菌染色を行う。これにより、従来法よりも簡易に蛋白質を含む試料中の微生物を生菌として測定できる。【選択図】なし
