METHOD FOR DETERMINING CONCENTRATION OF AUTO-ANTIBODIES SPECIFIC TO HUMAN HEPATIC F-PROTEIN IN BIOLOGICAL HUMAN FLUIDS CONTAINING SPECIFIC AUTO-ANTIBODIES
FIELD: medicine. ^ SUBSTANCE: method involves using high purity degree F-protein form to set solid body immunoenzyme analysis. The F-protein form is obtained from healthy human liver by exposing it to enzymatic hydrolysis to the extent the visible maceration signs being observable, then the tissue is treated with chelating agent and precipitated by centrifuging. Next to it, the cells are destroyed, cell elements are removed by centrifuging and F-protein is separated from cytosol by applying chromatography. The F-protein is then used for setting reaction. It is bound in covalent way to micropalette cells and reaction takes place that is used for determining hepatic pathologic changes. ^ EFFECT: wide range of functional applications. ^ 3 cl
