FRYER, BENJAMIN,LANIAUSKAS, DAINA,BLACKMOORE, MARCIA,WANG, HAIYUN,LILOVA, KOSTADINKA,NELSON, SHELLEY,ROSOCHA, ELIZABETH
申请号：
CA2928639
公开号：
CA2928639A1
申请日:
2014.05.21
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
The disclosure provides methods of preparing aggregated pluripotent stem cell clusters for differentiation to endoderm progenitor cells, pancreatic endocrine cells, mesoderm cells or ectoderm cells. Specifically, the disclosure provides methods of differentiating pluripotent cells into beta cell, cardiac cell and neuronal cell lineages using suspension clustering. The methods involve preparing the aggregated cell clusters followed by differentiation of these clusters.
