PROCEDURE FOR VIRUSES AND/OR PHYTOPLAZMS ELIMINATION FROM CONTAMINATED GRAPEVINE PLANTING MATERIAL, PLANTING MATERIAL OBTAINED BY SAID PROCEDURE AND ITS USE
SVEUČILIŠTE U ZAGREBU PRIRODOSLOVNO-MATEMATIČKI FAKULTET
发明人:
Leljak - Levanić, Dunja,Malenica, Nenad
申请号：
HRP20190444
公开号：
HRP20190444A2
申请日:
2019.03.05
申请国别(地区):
HR
年份:
2020
代理人:
摘要：
Izum se odnosi na postupak za uklanjanje virusa i/ili fitoplazmi iz zaraženog sadnog materijala vinove loze (Vitis vinifera) koji obuhvaća slijedeće korake: a) odabir ishodišnog tkiva； b) sterilizacija ishodišnog tkiva； c) izolacija i uvođenje eksplantata u kulturu tkiva； d) indukcija embriogeneze； e) sazrijevanje embrija； f) klijanje embrija i regeneracija biljaka； g) aklimatizacija biljaka na okolišne uvjete i rast na zemljanom supstratu u izoliranom sustavu, na sadni materijal vinove loze očišćen od virusa i/ili fitoplazmi dobiven ovim postupkom kao i njegovu uporabu u podizanju baznog nasada vinove loze.The invention relates to a method for removing viruses and/or phytoplasmas from infected grapevine planting material (Vitis vinifera) comprising the steps of: a) selecting a tissue source； b) sterilization of the tissue source； c) isolation and introduction of explants into tissue culture； d) induction of embryogenesis； e) embryo maturation； f) embryo germination and plant regeneration； g) acclimatization of plants to environmental conditions and growth on soil substrate in an isolated system, to a grapevine material cleaned of viruses and/or phytoplasmas obtained by this method as well as to its use in growing the grapevine base plantation.
