The present invention relates to a vaccine composition comprising a Staphylococcus aureus attenuated enterotoxin protein and cytotoxin protein, and more particularly to a Staphylococcus aureus enterotoxin protein, a Staphylococcus aureus cytotoxin protein, a vaccine composition for prevention of bovine mastitis, comprising the Staphylococcus aureus enterotoxin protein and Staphylococcus aureus cytotoxin protein and a method for preventing bovine mastitis comprising administering the vaccine composition to a bovine. The Staphylococcus aureus enterotoxin protein, the Staphylococcus aureus cytotoxin protein according to the present invention, and the vaccine composition comprising the proteins as an antigen can be used so that even vaccines comprising several antigens rather than all kinds of antigens show the excellent effects of prevention and treatment of bovine mastitis against all kinds of Staphylococcus aureus enterotoxin and cytotoxin having high incidence in Korea, thereby being more economically used for industrial purposes. Further, the vaccine composition for prevention of bovine mastitis, comprising the Staphylococcus aureus enterotoxin protein and Staphylococcus aureus cytotoxin protein according to the present invention has an excellent safety and bovine mastitis prevention and treatment effect even in the high CFU Staphylococcus aureus challenge test so that the composition can be variously utilized in Staphylococcus aureus vaccine and prevention related fields in future.La présente invention concerne une composition de vaccin comprenant une protéine d'entérotoxine atténuée et de cytotoxine de Staphylococcus aureus, et plus particulièrement une protéine d'entérotoxine de Staphylococcus aureus, une protéine de cytotoxine de Staphylococcus aureus, une composition de vaccin pour la prévention de la mammite bovine, comprenant la protéine d'entérotoxine de Staphylococcus aureus et la protéine de cytotoxine de Staphylococcus aureus et un procédé de prévention
