CASE WESTERN RESERVE UNIVERSITY;DENNIS, JAMES, E.;AWADALLAH, AMAD, A.;CAPLAN, ARNOLD, I.;WEIDENBECHER, MARK
发明人:
DENNIS, JAMES, E.,AWADALLAH, AMAD, A.,CAPLAN, ARNOLD, I.,WEIDENBECHER, MARK
申请号：
WO2007US85892
公开号：
WO2008082829(A2)
申请日:
2007.11.29
申请国别(地区):
世界知识产权组织国际局
年份:
2008
代理人:
摘要：
A hyaline-like, single layer cartilage tissue construct includes chondrogenic cells dispersed within an endogenously produced extracellular matrix. The single layer cartilage tissue construct has a glycosaminoglycan content substantially equal to the glycosaminoglycan content of native cartilage tissue. A method for generating a single layer cartilage tissue construct includes isolating a population of chondrogenic cells and then expanding the population of chondrogenic cells. Next, the population of chondrogenic cells is seeded into a bioreactor having a volume defined by oppositely disposed gas permeable membranes. The population of chondrogenic cells is then cultured in a serum- free culture medium for a time sufficient to permit the population of chondrogenic cells to differentiate and form the single layer cartilage tissue construct.
