The present invention relates a method for identifying and locating at least one nerve in a determined operation area. In the method, an image detector is utilized to scan the determined operation area according to a nerve direction forecast from a nerve identifying program, capture multiple 2D cross-sectional images of the determined operation area in different parts, and determine whether the 2D cross-sectional images belong to a target nerve by the nerve identifying program. If the 2D cross-sectional images are identified as the target nerve by the nerve identifying program, the nerve identifying program constructs a 3D nerve image from the 2D cross-sectional images by 3D image reconstruction.一種神經辨識定位方法,其用以辨識一待手術區域的至少一神經,該神經辨識定位方法是以一影像檢測器掃描該待手術區域,該影像檢測器並藉由一神經辨識程式提供的一預測神經走向掃描該待手術區域,並於該待手術區域的複數個區域擷取複數個二維截面影像,並以該神經辨識程式辨識該些二維截面影像是否為一待定位神經,當該神經辨識程式辨識該些二維截面影像是該待定位神經時,以一三維影像重建技術將該些二維截面影像,建構成一三維影像,該三維影像包含一三維神經影像。