SRINIVAS, MANGALA,KOSHKINA, OLGA,FIGDOR, CARL GUSTAV,DE VRIES, INGRID JOLANDA MONIQUE
申请号：
CA3017290
公开号：
CA3017290A1
申请日:
2017.03.13
申请国别(地区):
CA
年份:
2017
代理人:
摘要：
The invention relates to a process for the preparation of beads comprising a biocompatible hydrophobic polymer, a perfluorocarbon, polyvinylalcohoi and optionally a metal compound, comprising the steps of: a) adding the perfluorocarbon and optionally the metal compound to a solution of the biocompatible hydrophobic polymer in a polar solvent to provide a first liquid mixture, b) adding the first liquid mixture to an aqueous solution of a biocompatible surfactant comprising polyvinylalcohoi under sonication to obtain a second liquid mixture, c) maintaining the sonication of the second liquid mixture while cooling, d) evaporating the polar solvent from the second liquid mixture to obtain a suspension of beads comprising the biocompatible hydrophobic polymer, the perfluorocarbon and optionally the metal compound, e) separating the beads from the suspension and preparing a water suspension of the beads and f) freeze-drying the water suspension to obtain the beads, wherein the addition of the first liquid mixture to the biocompatible surfactant in step b) is performed within a period of at most seconds, wherein the sonication in step b) and the sonication in step c) are performed directly into the liquid mixtures by for example a probe or flow sonicator at an amplitude of at least 120 µm for 0.01-10 minutes and wherein the weight ratio of the biocompatible surfactant to the biocompatible hydrophobic polymer is at least 3:1. The invention further relates to beads having close F-H20 interactions, which are suitable for imaging purposes.
