IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE;THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD;THE UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGH;IMPERIAL INNOVATIONS LIMITED;DNA
发明人:
ERIC WALTER FREDERICK WOLFGANG ALTON,UTA GRIESENBACH,KAMILA MALGORZATA PYTEL,MICHAEL CHRISTIAN PAUL-SMITH,IAN ANDREW PRINGLE,STEPHEN CLARLES HYDE,DEBORAH REBECCA GILL,LEE ADRIAN DAVIES,ALAN CHRISTOPHE
申请号：
GB201409089
公开号：
GB2526339A
申请日:
2014.05.21
申请国别(地区):
GB
年份:
2015
代理人:
摘要：
Lentiviral gene transfer vectors pseudotyped with hemagglutinin neuraminidase (HN) and fusion (F) proteins from a respiratory paramyxovirus, comprising a promoter and a transgene but lacking an intron or introns positioned between said promoter and transgene are claimed. The lentiviral vector may be a HIV, SIV, FIV or EIAV or Visna/maedi vector. Alternatively claimed are methods of producing lentivirus comprising transfecting cells with plasmids encoding the lentivrus, adding a nuclease, harvesting the lentivirus, adding trypsin and purification. The use of said vectors in gene therapy, particularly for the treatment of respiratory tract diseases such as Cystic Fibrosis (CF) is claimed.
