A fluorescent scattering optical imaging system and method. The system comprises a laser (101), a micro-displacement table (102), an object table (103), at least one planar reflective mirror (104), a filter (105), a CCD camera (106) and a processor (107). An optical fiber head of the laser (101) is placed on the micro-displacement table (102). A reflecting surface of the planar reflective mirror (104) faces an object to be tested on the object table (103). The micro-displacement table (102) and the CCD camera (106) are electrically connected with the processor (107). The micro-displacement table (102) moves within a two-dimensional plane according to control signals of the processor (107). The laser (101) scans an area to be tested of the object to be tested which contains a fluorescent material to excite fluorescence. The CCD camera (106) is used for obtaining fluorescent images and laser images directly or on the basis of the reflection of the planar reflective mirror (104). The processor (107) is used for obtaining position information of the CCD camera (106), position information of the optical fiber head, position information of the planar reflective mirror (104), CT images or MRI images, fluorescent images and laser images of the object to be tested, so as to generate a three-dimensional fluorescent image of the area to be tested. The present invention can improve imaging quality and streamline system structure.Linvention a pour objet un système dimagerie optique diffuse à fluorescence et son procédé, lequel système comprend : un laser (101), une plateforme de microdéplacement (102), une platine porte-objet (103), au moins un miroir réfléchissant plat (104), un filtre optique (105), une caméra à dispositif à transfert de charge (106) et un processeur (107). Une tête de fibre optique du laser (101) se trouve sur la plateforme de microdéplacement (102). La surface réfléchissante du miroir réfléchissant plat (104) est orientée vers un objet à tester reposant sur
