The present invention discloses a machine (1) and a method for the automatic preparation of substances for intravenous application. Said machine comprises a container receiving zone (4) which defines a matrix of individual positions for initial and final containers (40,41), and a plurality of actuators (331, 332, 333) for transferring substances from initial container (40) to final container (41), each of said actuators (331, 332, 333) being positioned beneath said zone (4) for receiving initial and final containers, each of said actuators being able to move relatively, independently of the rest of the actuators, and each of said actuators being suitable for receiving and operating injectors (5) with different volumes and degrees of precision in order to remove substances from initial containers (40) and insert them into final containers (41).
