According to the invention, there is provided a novel soybean variety designated XB48E09. This invention thus relates to the seeds of soybean variety XB48E09, to the plants of soybean XB48E09 to plant parts of soybean variety XB48E09 and to methods for producing a soybean plant produced by crossing plants of the soybean variety XB48E09 with another soybean plant, using XB48E09 as either the male or the female parent.