Discloses an isolated promoter polynucleotide that comprises: a) the sequence of SEQ NO: 1 or 7 b) a sequence with at least 70% identity to the sequence of SEQ NO: 1 or 7 c) at least 50 contiguous nucleotides of the sequence of SEQ ID NO: 1 or 7 d) a sequence with at least 70% identity to the sequence of c) or e) the complement of anyone of a) to e) where the promoter polynucleotide is capable of controlling transcription of an operably linked polynucleotide in a plant. Also discloses a method for modifying expression in a plant cell or plant the method comprising transforming the plant cell with the polynucleotide generated by steps (a) to (e) as disclosed. Also disclosed are genetically modified plants, seeds or tissues, transformed by the method as disclosed. SEQ NO: 1 or 7 are derived from ryegrass (Lolium perenne cv. Bronsyn) promoter polynucleotides.
