A physiological signal detection clamp for being detachably disposed on a fabric worn by a living body, the physiological signal detection clamp includes: a body, two electrode units, a processing unit and a wireless transmission unit. Each of the two opposite side surfaces of the body has a fixing unit. The body is configured to clamp on the fabric by the two fixing unit. When the body is clamped on the fabric, the sensing surfaces of the two electrode units are correspondingly exposed on the outer side surface of the body and attach to the body surface of the living body for detecting the micro electric signal on the body surface of the living body. The processing unit transfers the micro electric signal detected by the electrode unit into a wireless transmission signal, and the wireless transmission unit outputs the wireless transmission signal.
