The invention relates to preparations of amniotic membrane-derived tissue grafts standardized for the concentration amniotic membrane and its constituent components by dry weight. The standardized amniotic membrane tissue graft is used by physicians and other healthcare providers in the surgical and minimally invasive medical therapy of a wide range of injuries and disease processes. The preparations maximize available quantities of non-cellular bioactive compounds and viscosity to enhance therapeutic efficacy for the desired application. The standardized amniotic membrane tissue graft preparations are semi-viscous fluids or gels which may be intraoperatively transplanted at the recipient site using a needless syringe, by non-operative percutaneous injection through a hypodermic needle, or by direct application.