The invention relates to preparations and methods of creating preparations of reconstituted amniotic membrane denuded of the amniocyte/epithelial cell layer to preserve amniocytes viability during processing. The tissue graft utilizes amniotic fluid as a source of additional viable stem cells. The amniotic tissue graft is flowable and injectable, and designed for use in surgical and minimally invasive medical therapy of injury and disease to promote tissue regeneration and healing. The tissue grafts optimize available quantities of viable mesenchymal stem cells and non-cellular bioactive compounds to enhance therapeutic efficacy. The tissue grafts are semi-viscous fluids which may be intraoperatively transplanted at the recipient site using a needless syringe or by non-operative percutaneous injection through a hypodermic needle.
