The invention provides extracts of Lao-Yiou tea which contain gallic acid and hesperidin. The preparation methods of the extracts of Lao-Yiou tea includes alcohol immersing method and ethyl acetate immersing method. The uses of the extracts of Lao-Yiou tea include preparing drugs for inhibiting human colorectal cancer cell growth and for anti-oxidation.本發明提供一種老柚茶萃取物,包含有沒食子酸(gallic acid)與桔皮苷(hesperidin),取得該老柚茶萃取物的方式包含乙醇浸泡法及乙酸乙酯法,該老柚茶萃取物的用途包含製備抑制人類大腸癌細胞生長之醫藥組成物及抗氧化組成物。