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POLIPEPTIDOS CON ACTIVIDAD FOSFOLIPASA C Y POLINUCLEOTIDOS QUE LOS CODIFICAN
专利权人:
NOVOZYMES A/S
发明人:
申请号:
ARP130104633
公开号:
AR093923A1
申请日:
2013.12.11
申请国别(地区):
AR
年份:
2015
代理人:
摘要:
The present relates to isolated polypeptides having phospholipase C activity, polynucleotides encoding the polypeptides. The application also relates to Nucleic Acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Claim 1: an isolated polypeptide that has activity of phospholipase C, selected from the group consisting of: (a) a polypeptide that has at least 90%, at least at least 91%, 93%, 92%, at least, at least at least 94%, 95%, 96% at least, at Least 97 at least%, 98%At least 99% or 100% Sequence identity with the mature polypeptide of sec id no. 2 (b) a polypeptide encoded by a polinucle\u00f3tido it hybridizes with very restrictive conditions (i) encoding the mature polypeptide sequence id no. 1 SEC, or (ii) The complement of length Pleta de I) (c) a polypeptide encoded by a polinucle\u00f3tido has at least at least 90%, 91%,At least at least 92%, 94%, 93%, at least, at least 95%, at least at least 96%, 97%, 98% at least, at least 99% or 100% Sequence identity with the mature polypeptide of sec id no. 1 (d) a variant of the mature Polypeptide The SEC ID no. 2 comprising a substitution, deletion and \/ or in Meeting in one or more positions and (e) a fragment of the polypeptide (a), (b),(c) or (d) has the activity of phospholipase C.La presente se relaciona con polipéptidos aislados que tienen actividad de fosfolipasa C, y polinucleótidos que codifican a los polipéptidos. La solicitud también se relaciona con constructos de ácidos nucleicos, vectores y células huésped que comprenden los polinucleótidos como así también métodos para producir y utilizar los polipéptidos. Reivindicación 1: Un polipéptido aislado que tiene actividad de fosfolipasa C, seleccionado del grupo que consiste en: a) un polipéptido que tiene al menos 90%, al menos 91%, al menos 92%, al menos 93%, al menos 94%, al menos 95%, al menos 96%, al menos 97%, al menos 98%, al menos 99% o 100% de identidad de secuencia c
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