OLINSKI, Robert Piotr,HOFF, Tine,OESTERGAARD, Peter Rahbek,SJOEHOLM, Carsten,PONTOPPIDAN, Katrine Fruergaard
申请号：
ES13701448
公开号：
ES2644007T3
申请日:
2013.01.25
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
Use of an isolated polypeptide having protease activity, selected from the group consisting of: (a) a polypeptide having at least 90% sequence identity with SEQ ID NO: 5 (B) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions or high stringency conditions with very: (i) the mature polypeptide coding sequence of SEQ ID NO: 1 (Ii) the coding sequence for the mature polypeptide of SEQ ID NO: 3 and / or (iii) the complementary strand full of (i) or (ii) (C) a polypeptide encoded by a polynucleotide having at least sequence identity of 90% with the sequence encoding the mature polypeptide of SEQ ID NO: 1 and / or SEQ ID NO: 3 (D) a variant comprising a substitution, deletion and / or insertion of one or more amino acids of SEQ ID NO: 5, wherein the number of substitutions, deletions and / or insertions in SEQ ID NO: 5 is not more than 19 and (e) a fragment of a polypeptide of (a), (b), (c) or (d) with protease activity, wherein the fragment comprises at least 169 amino acid residues in animal feed compositions and detergents, wherein the protease has at least 80% of the protease activity of SEQ ID NO: 5.Uso de un polipeptido aislado con actividad de proteasa, seleccionado del grupo que se compone de: (a) un polipeptido que tiene al menos un 90% de identidad de secuencia con SEQ ID NO: 5 (b) un polipeptido codificado por un polinucleotido que se hibrida en condiciones de astringencia alta o condiciones astringencia muy alta con: (i) la secuencia codificante del polipeptido maduro de SEQ ID NO: 1 (ii) la secuencia codificante del polipeptido maduro de SEQ ID NO: 3 y/o (iii) la cadena complementaria completa de (i) o (ii) (c) un polipeptido codificado por un polinucleotido que tiene al menos una identidad de secuencia del 90% con la secuencia codificante del polipeptido maduro de SEQ ID NO: 1 y/o SEQ ID NO: 3 (d) una variante que comprende una sustitucion, delecion y/o insercion de uno o mas aminoacidos de la SEQ ID NO: 5, en donde el
