The present invention discloses a thermal stable antibiofouling material and its preparation. The thermal stable antibiofouling material comprises a coating layer that uses hydrophobic interaction and π-interaction on surface of the substrate. The coating layer comprises a copolymer which is polymerized from a first monomer having an aromatic group and a second monomer having a zwitterionic group without ester group.本發明係關於一種熱穩定型抗生物分子沾黏材料及其製造方法，該熱穩定型抗生物分子沾黏材料包括一塗佈層和一基材，該塗佈層藉由包括疏水作用力與π作用力固定在該基材的表面上，其中上述之塗佈層包含一具有共振電子之芳香環的單體和一具有無酯類官能基之雙離子官能基的單體所聚合構成的一共聚物。其次，本發明亦提供該熱穩定型抗生物分子沾黏材料的製造方法。
