The present invention relates to bring out and keep the method for replying, have and be directed to the effect that breast carcinoma clinical remission patient brings out and keep protectiveness or therapeutic immunization for the peptide protectiveness cytotoxic T lymphocyte of HER2/neu proto-oncogene E75.This method comprise with optimal dose and scheme give patients effective dose comprise pharmaceutically suitable carrier, such as the adjuvant of recombined human GM-CSF and the vaccine combination of E75 peptide.Because also comprising, the E75 specific T-cells immunity that reduces, this method give annual or Booster vaccine dose half-yearly.The present invention also relates to be used for the vaccine combination of the method.
