The present invention relates to a non-thermal sterilization type microorganism culturing device, which uses ozone water to perform sterilization without heating a culturing tank for sterilization. The non-thermal sterilization type microorganism culturing device comprises: a culturing tank which receives culturing water and air and also has mediums injected therein to culture microorganism； a pipe which has a first pipe for introducing the culturing water from an exterior water storage unit, second and third pipes for supplying the introduced culturing water to the culturing tank, a fourth pipe for recovering the culturing water from the culturing tank to reinject the same to the second and third pipes or conveying microorganisms cultured in the culturing tank, and a fifth pipe for conveying microorganisms conveyed through the fourth pipe to a microorganism storage tank on the outside； a motor pump which is operated to pressure-feed culturing water through the pipe； an ozone generator which is installed to communicate with the second pipe on the second pipe and generates ozone for sterilization to supply the same to the inside of the culturing tank through culturing water passing through the second pipe； a medium container which is installed to accommodate a medium sterilized by radiation irradiation and communicate with the third pipe on the third pipe, and supplies the medium to the inside of the culturing tank through the culturing water passing through the third pipe； and a seed container which is coupled to the medium container while accommodating microorganism seed in order for the microorganism seeds to be supplied to the culturing tank with the medium together.COPYRIGHT KIPO 2019본 발명은 멸균 소독을 위해 배양탱크를 가열할 필요 없이 오존수를 이용하여 멸균 소독을 수행하는 비가열 멸균방식 미생물 배양장치에 관한 것이다. 본 발명은, 배양용수 및 에어의 공급과 더불어 배지가 투입되어 미생물을 배양하는 배양탱크； 배양용수를 외부의 용수저장부로부터 도입하는 제1관로와, 도입된 배양용수를 배양탱크로 공급하는 제2관로 및 제3관로와, 배양탱크로부터 배양용수를 회수하여 제2관로 및 제3관로로 재투입시키거나 배양탱크 내에서 배양 완료된 미생물을 이송하는 제4관로와, 제4관로를 통해 이송되
