Paul KEMP,David SHERING,Andrew SHERING,Penny JOHNSON,Damian MARSHALL
申请号：
US15153499
公开号：
US20160333320A1
申请日:
2016.05.12
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Disclosed is a method of preparing a collagenous construct comprising (i) casting viable collagen-producing human dermal fibroblasts in a support matrix comprising fibrin onto a support material, (ii) incubating in situ said support matrix with said viable collagen-producing human dermal fibroblasts in a collagen-inducing medium thereby (a) inducing or enhancing collagen production by said viable collagen-producing human dermal fibroblasts to form a collagenous construct, and (b) degrading said fibrin, (iii) rendering said collagenous construct free of said viable collagen-producing human dermal fibroblasts, and (iv) cross-linking said collagenous construct with a chemical or exposure to ultraviolet light.
