An object of the present invention is to provide a method for sterilizing an object to be washed in a commercial washing machine, which can surely sterilize microorganisms including Bacillus cereus using chlorine dioxide when washing the object to be washed, a sterilizing apparatus, and an operation. Provided is a commercial washing machine using a method for sterilizing an object to be washed of a washing machine for business use. The present invention relates to a method for disinfecting an object to be washed, which is used in a washing machine 2 for washing an object to be washed 1 and sterilizes the object to be washed 1 in a washing tub 3 of the washing machine 2. A chlorine dioxide generating step 4 of mixing water and a plurality of chemicals A, B, and C to generate a high concentration of chlorine dioxide; and a washing tub of the high concentration chlorine dioxide generated in the chlorine dioxide generating step 4. And an injection diluting step 5 for diluting the object to be washed 1 in the washing tub 3 and diluting it to a predetermined concentration with washing water. [Selection diagram] Fig. 1【課題】本発明は、被洗物を洗濯する際に、二酸化塩素を用いてセレウス菌を含む微生物の確実な殺菌を行うことができる業務用洗濯機の被洗物殺菌方法、殺菌装置及び業務用洗濯機の被洗物殺菌方法を用いた業務用洗濯機を提供する。【解決手段】本発明は、被洗物1を洗濯する洗濯機2に用いられて、洗濯機2の洗濯槽3内で被洗物1を殺菌処理する洗濯機用の被洗物殺菌方法であって、水と複数の薬剤A、B、Cとを混合して高濃度の二酸化塩素を生成する二酸化塩素生成工程4と、二酸化塩素生成工程4で生成された高濃度の二酸化塩素を洗濯槽3内に注入し洗濯槽3内で被洗物1を洗濯する洗濯水により所定の濃度に希釈する注入希釈工程5と、からなる。【選択図】 図1