How to release sporocysts from oocysts using controlled shear force

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专利权人:

エンブレックス・インコーポレイテッド

发明人:

ジェイムズ・アール・ハッチンス,ケリアン・ウィルソン,アンジェラ・ハートマン,ケリー・ミシェル・ハリス

申请号：

JP2009549077

公开号：

JP5690488B2

申请日:

2008.01.29

申请国别(地区):

JP

年份:

2015

代理人:

摘要：

Methods of releasing sporocysts/sporozoites from oocysts are provided wherein a solution of oocysts is subjected to controlled shear forces sufficient to rupture the oocysts walls and release sporocysts/sporozoites therefrom. A solution of oocysts is passed through a Microfluidizer(R) processor chamber unit under defined conditions of chamber diameter, chamber geometry, and pressure. Oocysts impact the wall of the chamber and are subjected to controlled, high shear forces, tearing open the oocyst wall and releasing the sporocysts/sporozoites intact.

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中国工程科技知识中心

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