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METHODS OF RELEASING SPOROCYSTS FROM OOCYSTS USING CONTROLLED SHEAR FORCES
专利权人:
发明人:
KELLY MICHELLE HARRIS,ANGELA HARTMAN,JAMES EARL HUTCHINS,KERRIANNE WILSON
申请号:
IN4965/DELNP/2009
公开号:
IN264100B
申请日:
2009.07.30
申请国别(地区):
IN
年份:
2014
代理人:
摘要:
Methods of releasing sporocysts/sporozoites from oocysts are provided wherein a solution of oocysts is subjected to controlled shear forces suffcient to rupture the oocysts walls and release sporocysts/sporozoites therefrom. A solution of oocysts is passed through a Microfluidizer® processor chamber unit under defined conditions of chamber diameter, chamber geometry, and pressure. Oocysts impact the wall of the chamber and are subjected to controlled, high shear forces, tearing open the oocyst wall and releasing the sporocysts/sporozoites intact
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