PROBLEM TO BE SOLVED: To provide a method for producing a frozen cell sheet which can not be cracked or broken even when it is frozen and can be conveniently frozen for preservation.SOLUTION: There is disclosed a method for producing a frozen cell sheet in which a cell sheet coated with a vitrified solution containing a cell-permeable cryoprotective agent is frozen in a gas phase environment at -20°C to -269°C.COPYRIGHT: (C)2013,JPO&INPIT【課題】凍結させてもひび割れたり破りたりせず、かつ簡便に凍結保存が可能な凍結細胞シートの製造方法の提供。【解決手段】細胞浸透性の凍害保護剤を含むガラス化液で被覆された細胞シートを、-20℃~-269℃の気相雰囲気中で凍結する凍結細胞シートの製造方法。【選択図】なし
