A functional composition for prevention and anticancer comprising edible carbohydrates and polyacetylene components isolated from Panax ginseng as an efficient component and preparation method thereof
The present invention separates polyacetylene from Korean ginseng using flash column chromatography and thin layer chromatography (TLC) and forms a complex with edible carbohydrate to treat cervical cancer (HeLa) cells to measure cytotoxicity, The complex is identified by mass spectrometry, FT-IR analysis and nuclear magnetic resonance spectroscopy, and the complex is selected from the polyacetylene component falcarinol and the edible carbohydrates α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, maltodextrin and pullulan There is an excellent effect of providing cancer prevention and anti-cancer immunity-functioning health functional food and animal feed composition as well as pharmaceutical composition for prevention and treatment of cancer containing as active ingredient.본 발명은 flash column chromatography 및 박층 크로마토그래피법(TLC)을 사용하여 고려인삼으로부터 폴리아세틸렌 성분을 분리하고 식용 탄수화물과 복합체를 형성시켜 자궁경부암(HeLa) 세포에 처리하여 세포 독성을 측정하고 가스 크로마토그래피 질량분석과 FT-IR 분석 및 핵자기공명 분광 분석을 통하여 복합체를 확인하고 상기 폴리아세틸렌 성분 falcarinol과 식용 탄수화물 α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, maltodextrin 및 pullulan 중에서 선택되는 어느 하나의 복합체를 유효성분으로 함유하는 암예방 및 치료용 약학적 조성물 뿐만 아니라 암예방 및 항암 면역 기능성 건강기능식품 및 동물사료 조성물을 제공하는 뛰어난 효과가 있다.
