The present invention discloses a method of combining pressure and enzyme to extract a hydrolysate of a non-animal-derived composition. The method comprises: adding an enzyme into a non-animal biological raw material, and proceeding hydrolyzation at a pressure of 0.2-600MPa, so as to obtain a hydrolyzed extract.本發明揭露一種結合壓力與酵素萃取非動物性成份水解物之方法,該方法包含:將酵素加入非動物生物原料中,並於0.2~600MPa之壓力下進行水解,進而取得水解萃取物。