PURPOSE: A method for plantlet formation of blueberry cv. toro, legacy or oregonblue is provided to increase the number of plantlets during a growth culturing process since a multi-shoot formation rate is higher than a meristem-tip culture. CONSTITUTION: A blueberry leaf is taken into a medium(1) to induce an initial callus and multi-shoot. 1 to 3 mg/L of Zeatin Riboside, 30 to 60 mg/L of FeNa-EDTA, 50 to 150 mg/L of myo-inositol, 1 to 3 mg/L Thiamine HCl, 0.5 to 1.5 mg/L of Nicotinic acid, 0.5 to 1.5 mg/L of Pyridoxine HCl, 10 to 30 g/L of sucrose, and 4 to 8 g/L of agar are added to the medium and then cultured in a next medium(2) to form a plant body. In the next medium, Zeatin Riboside is added at 1/10 of the above concentration or not added, or the medium(2) is cultured in a medium added with 0.1 to 3 mg/L of IBA(Indole-3-Butyric Acid) to induce formation of rood by circulating from a culturing chamber to a greenhouse. The medium(1) includes 1 to 3 mg/L of zeatin, 30 to 60 mg/L of FeNa-EDTA, 50 to 150 mg/L of myo-inositol, 10 to 30 g/L of sucrose, and 4 to 8 g/L of agar. [Reference numerals] (AA) 14 weeks； (BB) 18 weeks； (CC) 24 weeks； (DD) 34 weeks； (EE) 3 months본 발명은 엽편배양 방법을 이용한 블루베리 품종의 기내 유식물체 형성 방법에 관한 것으로서, 구체적으로, 본 발명자들은 불루베리의 조직배양묘 생산 기술을 확립하고자 노력하던 중, 국내 적응성이 뛰어나고 비교적 많은 재배면적을 차지하는 하이부시 계통 2품종(토로, 레가시)과 래빗아이 계통 1품종(오레곤블루)을 엽편배양하여 기내에서 8~9개월 내에 유식물체를 형성하는 배지 조성 및 배양조건을 개발하였고, 이를 블루베리 국산 조직배양 묘목의 확대 보급을 위한 원천기술로 활용할 수 있음을 확인하였다.
