The present invention provides a method for aseptically culturing fruiting bodies of antrodia cinnamomea, comprising: inoculating strains of antrodia cinnamomea in a sterilized agar medium, and carrying on subculture to produce secondary myceliums and fruiting bodies of antrodia cinnamomea.一種培養牛樟芝子實體之方法,係包括:將牛樟芝菌種接菌至經滅菌之洋菜培養基,進行繼代培養,以產生次級菌絲體,並於該繼代培養過程中篩選出無變種及無毒性的次級菌絲體,以及,以該經篩選出無變種及無毒性的次級菌絲體進行該繼代培養,以產生子實體。