Provided is a recombinant Herpes simplex virus. The recombinant Herpes simplex virus does not express functional ICP0 protein and ICP34.5 protein; but expresses functional UL43 protein, functional UL41 protein (i.e. vhs protein), functional UL48 protein (i.e. VMW65 protein) or any combination thereof. Further provided are a virus vector constructed on the basis of the recombinant Herpes simplex virus, a pharmaceutical composition comprising the recombinant Herpes simplex virus or the virus vector, and use of the recombinant Herpes simplex virus or the virus vector.