Utilisation de substances réduisant la teneur cellulaire en glutathion pour la fabrication d'un médicament pour le traitement de maladies auto-immunes transmises par cellules T
ROECKEN, MARTIN,GHORESCHI, KAMRAN,WEIGERT, CHRISTINA MATHILDE
申请号：
EP11008821.8
公开号：
EP2428208A2
申请日:
2007.10.31
申请国别(地区):
EP
年份:
2012
代理人:
摘要：
Die vorliegende Erfindung betrifft eine Verfahren zur Bestimmung des Potentials von pharmazeutischen Substanzen, den zellulären Glutathiongehalt zu senken und/oder den zellulären ROS-Gehalt zu steigern； die derart identifizierten Substanzen, die den zellulären GSH-Level absenken, können zur Behandlung von T-Zell vermittelten Autoimmunkrankheiten eingesetzt werden.Use of substances for reducing cellular glutathione content for producing a medicament for treating T-cell mediated autoimmune diseases, where the substances are L-buthionine-S,R-sulfoximine, ifosfamide, diethyl maleate or acetaminophen, is claimed. Independent claims are included for: (1) determining the potential of pharmaceutical substances for reducing the cellular glutathione content and/or for increasing the cellular reactive oxygen species content comprising either incubating the cells with different concentrations of substances and determining the glutathione content of the cells in comparison to the control cells； or incubating the cells with different concentrations of substances and staining the cells with at least a fluorescent- or chemiluminescent-dye, preferably with 2',7'-dichlorodihydrofluorescein diacetate； and (2) determining the genetic susceptibility for developing a autoimmune disease comprising extracting the peripheral blood leucocytes, isolating the genomic DNA from the peripheral blood leucocytes and detecting the genotype of the glutathione-S-transferase by real-time polymerase chain reaction and/or gene-chips. ACTIVITY : Immunosuppressive； Neuroprotective； Antidiabetic； Antiarthritic； Antirheumatic； Antipsoriatic； Antiinflammatory； Gastrointestinal-Gen； Antiulcer； Dermatological； Antibacterial； Antiasthmatic； Antiallergic. MECHANISM OF ACTION : Interleukin-12 inhibitor； Interleukin-23 inhibitor.
